Microbial Degradation And Nutrient Optimization Of Pulp -Books Download

MICROBIAL DEGRADATION AND NUTRIENT OPTIMIZATION OF PULP
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International Research Journal of Engineering and Technology IRJET e ISSN 2395 0056. Volume 03 Issue 08 Aug 2016 www irjet net p ISSN 2395 0072. 2 3 Microorganism 3 RESULTS AND DISCUSSION, The species selected for treatment were Rhizopus sp. The effluent was collected and analyzed as per standard. and Bacilus subtilis Bacterial species was collected from. methods of analysis of water and wastewater The, Microbial Culture Centre Pune Fungal species was collected. characterization studies were conducted for pH turbidity. from Microbiology Lab of College of Engineering, colour BOD and COD Table 1 shows the results of the. Trivandrum,analysis of the samples, 2 4 Treatment From the study it is observed that the effluent is. highly coloured The values of BOD COD were very high as. The species selected for treatment were Rhizopus compared to CPCB limits for discharge into inland surface. sp and Bacilus subtilis PDA medium and PDB medium for water The dark brown colour reduces the DO content. culture of fungus was prepared Nutrient agar and nutrient and sunlight which affects aquatic life These. broth for culture of bacteria was prepared Cultures were compounds are not easily degraded naturally which. prepared on agar slants and transferred to broth for demands the use of various fungal bacterial algal species. culturing them in waste water sample and enzymes for the effective removal of colour turbidity. COD and BOD,A batch set up was done for treating the waste.
water sample Collected sample were taken in conical flasks Table 3 1 characteristics study of effluent. and analysed Then it was supplemented with nutrients to. improve the efficiency of the microorganisms The nutrients Sl no Parameter Values Tolerance limit for. used are glucose and NH4Cl which are supplemented at discharge into inland. concentration ranging from 0 25 1 Then it was treated surface water CPCB. using both fungal and bacterial species individually Analysis 2012. was done after 2 4 6 8 and 10th day for both BOD and COD. Variation in colour was determined by finding the variation 1 pH 7 31 5 5 9. in absorbance using spectrophotometer,2 Colour brown Colourless. 3 Turbidity NTU 100 5,4 BOD mg L 1110 30,5 COD mg L 12800 250. 3 1 BOD and COD reduction,Table 3 2 Variation in BOD with fungal treatment. Fig 1 Slant prepared for bacterial culture and fungal culture. TIME BOD mg L,1 0 5 0 25,nutrient nutrient nutrient. 2 nd day 760 620 820,4 th day 595 5 535 696 6,6 th day 356 254 408 9.
8 th day 200 98 86 127 8,10 th day 98 52 4 60,Fig 2 Batch setup. 2016 IRJET Impact Factor value 4 45 ISO 9001 2008 Certified Journal Page 1920. International Research Journal of Engineering and Technology IRJET e ISSN 2395 0056. Volume 03 Issue 08 Aug 2016 www irjet net p ISSN 2395 0072. Table 3 3 Variation in COD with fungal treatment The tables 3 2 and 3 3 show the reduction in BOD. and COD after fungal treatment The tables 3 4 and 3 5. TIME COD mg L shows the reduction in BOD and COD after and bacterial. treatment The results obtained after treatment with the. 1 0 5 0 25 fungal and bacterial species showed reduction in BOD COD. nutrient nutrient nutrient and colour, 2 nd day 8697 9025 10000 COD reduces from second to tenth day by 90 2 by. fungal treatment and 88 6 by bacterial treatment The. 4 th day 6020 7557 8967 reduction in COD is more when the sample is supplemented. with 0 5 nutrients and inoculated with fungal species But. 6 th day 4500 5940 6567 for sample inoculated with bacterial species the reduction is. more when the sample is supplemented with 1 nutrients. 8 th day 2105 2589 3700 BOD reduction also occurs after treatment with both. bacteria and fungus BOD reduces from second to tenth day. 10 th day 987 1200 2000 by 90 4 by fungal treatment and 87 6 by bacterial. treatment The reduction in BOD is more when the sample is. supplemented with 0 5 nutrients and inoculated with. fungal species But for sample inoculated with bacterial. Table 3 4 Variation in BOD with bacterial treatment species the reduction is more when the sample is. supplemented with 1 nutrients,TIME BOD mg L,1 0 5 0 25. nutrient nutrient nutrient,2 nd day 730 761 870,4 th day 500 540 4 668. 6 th day 256 681 312 400,8 th day 97 3 112 150,10 th day 86 94 112 9.
Fig 3 1 Variation in BOD after fungal treatment with. different nutrient concentration, Table 3 5 Variation in COD with bacterial treatment. TIME COD mg L,1 0 5 0 25,nutrient nutrient nutrient. 2 nd day 8500 7156 9200,4 th day 6025 5985 7560,6 th day 4758 3250 400. Fig 3 2 Variation in BOD after bacterial treatment with. 8 th day 1850 1207 2750,different nutrient concentration. 10 th day 950 700 1000, 2016 IRJET Impact Factor value 4 45 ISO 9001 2008 Certified Journal Page 1921.
International Research Journal of Engineering and Technology IRJET e ISSN 2395 0056. Volume 03 Issue 08 Aug 2016 www irjet net p ISSN 2395 0072. Fig 3 3 Comparison between variations in BOD with Fig 3 6 Comparison between variations in COD with. fungal and bacterial treatment fungal and bacterial treatment. The figure 3 1 shows the variation in BOD while The figure 3 4 shows the variation in COD while. treating with fungal species The reduction is more when the treating with fungal species The reduction is more when the. effluent is supplemented with0 5 concentration of effluent is supplemented with0 5 concentration of. nutrients The figure 3 2 shows the variation in BOD while nutrients The figure 3 5 shows the variation in COD while. treating with bacterial species The reduction is more when treating with bacterial species The reduction is more when. the effluent is supplemented with 1 concentration of the effluent is supplemented with 1 concentration of. nutrients The figure 3 3 shows comparison between nutrients The figure 3 6 shows comparison between. variations in BOD with fungal and bacterial treatment By variations in COD with fungal and bacterial treatment By. comparing the values it is seen that fungus is more capable comparing the values it is seen that fungus is more capable. of reducing BOD compared to bacterial species This is due to of reducing COD compared to bacterial species This is due to. increasing reduction of lignin derivatives and organic matter efficient reduction of lignin derivatives and high. by fungal species molecular weight compounds by fungal species. 3 2 Colour,Reduction in absorbance occurs with bacterial and. fungal treatment Absorbance reduces from first day to. tenth day by 73 by fungal treatment and 67 46 for, bacterial treatment Maximum reduction is seen at 0 5. concentration of nutrients for fungal treatment, Theemaximum reduction is seen at 1 concentration of. nutrients for bacterial treatment The presence of brown. Fig 3 4 Variation in COD after fungal treatment with colour in the effluent is mainly due to lignin derivatives. different nutrient concentration Fungus is more efficient in reducing these lignin derivatives. so it shows better colour reduction,4 CONCLUSIONS,Pulp and paper mill effluent contains harmful. chemicals lignin and if discharged untreated into the surface. water bodies or land cause serious environmental damages. The physical and chemical methods used for the treatment of. the pulp and paper mill effluent are not economic and not. very efficient in reducing lignin So biological methods are. more preferred Microorganisms like fungi bacteria etc can. be used for treatment, Fig 3 5 Variation in COD after bacterial treatment with In the present study the effluent was treated with a.
different nutrient concentration bacterial species as well as fungal species The samples were. also supplemented with Glucose and NH4Cl at 1 0 5 and. 2016 IRJET Impact Factor value 4 45 ISO 9001 2008 Certified Journal Page 1922. International Research Journal of Engineering and Technology IRJET e ISSN 2395 0056. Volume 03 Issue 08 Aug 2016 www irjet net p ISSN 2395 0072. 0 25 concentration The average COD reduction is 90 2. by fungal treatment and 88 65 by bacterial treatment The. average BOD reduction is 90 4 by fungal treatment and. 87 6 by bacterial treatment Colour reduction is 73 by. fungal treatment and 67 46 reduction for bacterial. Addition of nutrients has enhanced the rate of reduction. of BOD COD and colour Optimum nutrient concentration for. fungal species is 0 5 and bacterial sp is 1 as they show. maximum reduction when supplemented with the nutrients. at the mentioned percentages Compared to bacterial. species the fungal species is more effective in reducing BOD. COD and colour Fungus has the capacity to degrade lignin. derivatives and organic matter much efficiently compared to. REFERENCES,1 Anjali Singhal Indu Shekhar Thakur 2009. Decolourization and detoxification of pulp and, paper mill effluent by Cryptococcus sp Biochemical. Engineering Journal vol 46 pp 21 27,2 Gomathi V Cibichakravarthy B Ramanathan A. Sivaramaiah Nallapeta Ramanjaneya V R Mula,Jayasimha Rayalu 2012 Decolourization of paper. mill effluent by immobilized cells of Phanerochaete. chrysosporium International Journal of,Plant Animal and Environmental Sciences vol 2.
pp 141 146,3 Jacob Varughese Vadakenat S Saravana Babu 2014. Comparitive study on the efficiency of bacterial sp. And fungal sp In pulp and paper industry effluent,remediation International Journal of Research in. Chemistry and Environment vol 4 pp 63 68, 4 Lokeshwari N Srinikethan G Joshi S G Shasikala I Sri. kanth B Bashirahmed Sushema L 2013 Isolation, of fungi for delignification of Pulp and paper mill. effluent International Journal of current,Engineering and Technology pp124 128.
5 Pratibha S Indu S T 2006 Colour removal of, anaerobically treated pulp and paper mill effluent. by microorganisms in two steps bioreactor,Bioresource Technology Vol 97 pp218 223. 6 Radhakrishnan Saraswathi Manghatai Kesavan,Saseetharan 2010 Investigation on. microorganisms and their degradation efficiency in. paper and pulp mill effluent Journal of Water,Resource and Protection vol 2 pp 660 664. 7 Sharma R Subash c Amrita s Kriti S 2014,Degradation of Pulp and Paper Mill Effluents The.
institute of Integrative Omics and Applied,Biotechnology Journal Vol 5 pp 6 12. 2016 IRJET Impact Factor value 4 45 ISO 9001 2008 Certified Journal Page 1923.


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